Table 4

Kinetic parameters	Bacillus Strain	Pre optimization		Post Optimization
Kinetic parameters	Bacillus Strain	Parent	BSU-5	Parent	BSU-5
Substrate Consumption parameters	^Aµ(h^-1)	0.105	0.176	0.131	0.182
	^BYx/s	0.46	5.677	1.02	9.50
	^CYp/x,	2.13	21.98	4.99	28.1
	^Dqs(g/g/h)	0.19	0.63	0.20	0.123
	^EQs(g/L/h)	1.125	0.16	0.96	0.355
Product Formation Parameters	^FQx(g/L/h)	0.244	0.505	0.196	0.120
	^GYp/s	0.217	0.258	0.204	0.338
	^Hqp (Product/h/g)	0.089	0.916	0.208	1.17
	^IQp (Product/L/h)	0.5225	2.86	0.98	3.38

Enzyme were produced in basal medium contains initial sugar concentration is 50 g/L (w/v).
^Aμ(h^-1), Specific Growth rate
^BYx/s, Enzyme produced/g glucose consumed
^CYp/x, Enzyme produced/g cell formed
^Dqs(g/g/h), g sugar consumed/ g cells/h
^EQs(g/L/h), g sugar consumed/L/h
^FQx (g/L/h), g cells formed/L/h
^GYp/s, Cell formed/g sugar consumed
^Hqp (Product/g/h), Enzyme produced/ g cells/ h
^IQp(Product/L/h), Enzyme produced/L/h

Table 4: Comparison of Kinetic parameters for alkaline protease production among parent Bacillus subtilis M-9 and selected Bacillus subtilis BSU-5 mutant in submerged fermentation.