Bacterial culture Acetylene added Rate
µg N2 h-1 mg dry wt cells-1 µg N2O h-1 mg dry wt cells-1
Pseudomonas aeruginosa No 22.6 ± 6.8a,b 35.5 ± 10.7 a,b,c
Pseudomonas aeruginosa Yes UDd 25.8 ± 3.3a,b
Pseudomonas chloroaphis No UDd 2.9 ± 1.0b,e
Pseudomonas chloroaphis  Yes UDd 2.3 ± 1.1b,e
amean of five determinations; bBased on the results of t-tests (p > 0.05), we failed to reject the null hypothesis that the means of the rates obtained the presence and absence of acetylene with either P. aeruginosa (single underline) and P. chloroaphis (double underline) were the same; cValue was not measured directly as N2O was not produced. The rate was obtained by converting the rate, measured by the production of N2, to N2O h-1 mg dry wt cells-1; dUD, unable to determine as N2 was not produced. emean of triplicate determination
Table 2: Denitrification enzyme activity rate (± Std Dev) of bacterial cultures determined by measuring accumulation of the nitrogen or nitrous oxide end product by membrane inlet mass spectrometry.