Purification steps |
Total proteins
mg/ml |
Total activity
U |
Specific activity (U/mg) |
Fold purification |
% yield |
Crude extract |
6024.28 |
361456 |
60 |
1 |
100 |
Precipitation |
620.42 |
359843 |
580 |
9.66 |
99.55 |
DEAE-cellulose chromatography |
160.14 |
316436 |
1976 |
32.99 |
87.54 |
Sephadex G-75 chromatography |
70.86 |
271748 |
3835 |
63.91 |
75.18 |
|