| Hydrolysis agent |
Hydrolysis Conditions |
Additives |
Specific
Determination |
| 6 M HCl |
110°C, 24 h |
0.02% Phenol |
All residues except of Cys, Trp |
| 6 M HCl or 4 M MSA |
110°C, 24 h |
0.2% Sodium azide |
Cys |
| 6 M HCl |
110°C, 18 h |
5% Thioglycolic acid, 0.1% phenol, 3,3’-dithiodipropionic acid |
Cys |
| 6 M HCl |
|
3-Bromopropylamine |
Cys |
| 6 M HCl |
145°C, 4 h |
Samples previously oxidized with performic acid |
Cys, Met, Lys |
| 4 M MSA |
|
3-(2-aminoethyl)indole |
Trp, Methionine sulfoxide |
| 4 M MSA |
115°C, 22 h |
Samples previously alkylated, tryptamine |
All residues |
| 4 M MSA |
160°C, 45 min |
|
All residues |
| 4 M MSA or 5.7 M HCl |
150°C, 90 min |
Oxidation with performic acid |
All residues |
| 50°C, 10 min |
| 3 M p-Toluenesulfonic acid |
|
|
Methionine sulfoxide |
| 12 M HCl–propionic acid (1:1) |
150°C, 90 min |
|
Resin-bound peptides |
| 12 M HCl–propionic acid (1:1) |
840 W, 1–7 min microwave |
|
Resin-bound peptides |
| p-Toluenesulfonic acid |
15 min, microwave |
|
|
| DCl |
medium power, 30 min microwave |
|
Sensitive residues |
| 2.5 M Mercaptoethanesulfonic acid |
176°C, 12.5 min |
S-Pyridylethylated samples |
Cys, Trp |
| 6 M HCl–TFA (6:3) |
120°C, 16 h |
Dithiodiglycolic acid, 1% phenol |
Cys |
| HCl |
|
Thioglycolic acid |
Trp |
| HCl |
110°C, 24 h |
0.4% β- Mercaptoethanol |
Trp |
| HCl |
166°C, 25 min or 145°C, 4 h |
3% Phenol |
Trp |
| HCl |
145°C, 4 h |
Tryptamine |
Trp |
| 6 M HCl |
145°C, 4 h gas phase |
Tryptamine[3-(2-aminoethyl)]indole |
Trp |
| TFA–HCl (1:2) |
166°C, 25-50 min |
5% Thioglycolic acid |
Trp, Met |
| 7 M HCl, 10% TFA |
|
10% Thioglycolic acid, indole |
Trp |
|
