Parameters Classical LB 4I8S 4IAT Resolution Range (Å) 2.00 – 40.17 (2.00 – 2.11) 2.00 – 39.39 (2.00 – 2.11) a = b, c (Å) 80.34 37.16 78.78 36.99 α = β = γ (°) 90 90 Completeness (%) 100.0 100.0 Mosaicity 0.97 0.54 Spacegroup P 43 21 2 I/Sigma 12.1 (8.6) 31.8 (20.0) Rsym 0.199 0.066 Rfactor 0.195 0.175 Rfree 0.259 0.241 Total number of reflections 8200 7903 R.m.s. on bond length (A) 0.022 0.022 R.m.s. on angles (°) 1677 1797

Table 1: Statistics of the X-ray diffraction studies of the two lysozyme crystals, the former growth with classical hanging drop method and the latter growth with LB modified vapour.