| Frequency shift, 1/cm |
Bond vibration |
Residue in lysozyme |
Sensitivity |
| 505 |
Disulfide bond S-S |
There are four pairs of S-S bonds in lysozyme. Two of them S6-S127 and S30-S115 are close to the C-end |
Increase in peak intensity corresponds to the increase in the amount of S-S bonds in lysozyme |
| 634 |
C-S streching mode in Cys residues |
Cys |
Amount of Cys residues |
| 760 |
Indole ring breathing mode of Trp |
Trp (there are 6 Trp in lysozyme, one of them Trp123 locates closely to S6-S127, S30-S115 in C-end) |
Sensitive to the hydrophobic invironment. Characteristical peak of lysozyme |
| 865-883 |
N1H site of Trp |
Trp |
Frequency of the band depends on the strength of H-bond between Trp and other side-chain amino-acid residue. 865-871 1/cm – in H non-bond Trp; 883 – in high H-bonding strength Trp residues |
| 890, 930 |
Backbone C(alpha)-C, C(alpha)-N |
Peptide backbone |
|
| 1001 |
Ring breathing of Phe |
|
|
| 1010-1014 |
Ring breathing mode of Trp |
Trp |
|
