Sample Number of reads Number of taxa
Total Identified* Unidentified†
Identity <97.0% Length <231 base
Saliva 15,581 15,339 215 27 249
Tongue coating 298,079 288,953 8,975 151 182
*Identification of each read was performed by BLAST search against reference sequences of Human Oral Microbiome Database with ≥97.0% identity over an alignment of ≥ 231 bp
†Reads that did not meet the criteria for identification were assigned to unidentified reads
Data are expressed as the total number of reads or taxa in pooled samples from all 20 subjects in the two groups
Table 3: Summary of pyrosequencing analysis.