Figure 2: (a) SDS-PAGE of purified L-aao. Lane 1 – Purified A. fumigatus L-amino acid oxidase, lane 2- Crude enzyme, lane 3- Standard protein ladder (Prestained Molecular weight marker MW 27,000-180,000; Sigma). (b) Molecular weight determination by size exclusion chromatography on Sephadex G-75. The molecular weight standards used were glucose oxidase (Ve/V0=1.38); fructose 6-phosphate kinase (Ve/V0=2.72); ovalbumin (Ve/V0=3.22) and carbonic anhydrase (Ve/V0=4.11). Ve/V0 of A. fumigatus L-aao=2.55. (c) The purity of the L-aao was determined by reverse-phase C18 HPLC column (Novapack, 3.9 x 150 mm). The peak at retention time 6.322 signifies the purified enzyme.