Figure 3: (a) Effect of buffer pH on the L-aao activity. (The experiment was done in triplicate and the data are expressed as mean ± standard deviation). (b) Substrate saturation kinetics of A. fumigatus L-aao. (c) Lineweaver Burk plot of A. fumigatus L-aao. The intercept on the x-axis i.e. -1/Km= -0.023 and the intercept on y-axis i.e. 1/vmax = 23. Therefore Km= 43.47 and Vmax =0.0434 µmol/min/mL. (d) Effect of metal salts on the L-aao activity of A. fumigatus. The metal salts were added in 10-4 and 10-3 M concentration during the enzyme reaction.