| Step |
Vol.(mL) |
Total A.(U) |
Total Prot. (mg) |
S.A.(U/mg) |
Yield (%) |
Purif.
fold |
| Crude |
40 |
1.23 |
96.8 |
0.012 |
100 |
1 |
| ASF |
6 |
0.92 |
38.0 |
0.024 |
74.80 |
2 |
| IEC |
14 |
0.30 |
7.0 |
0.043 |
24.40 |
3.6 |
| GFC |
12 |
0.23 |
1.9 |
0.121 |
18.70 |
10 |
ASF- ammonium sulphate fractionation; IEC- Ion Exchange Chromatography on DEAE Sephadex A-50; GFC- gel fractionation chromatography on Sephadex G-75 A. = Activity; S.A. = Specific Activity |
