Figure 5: The evolutionary history was inferred using nearly complete ITS sequences (~600 bp) using ITS 4 and 5 primers from 15 Trichoderma isolates and available NCBI sequences, constructed by Neighbor Joining method [11]. The optimal tree with the sum of branch length = 0.36770521 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown above the branches [21]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 600 positions in the final dataset. Phylogenetic analyses were conducted in MEGA4 [12]. The scale indicates evolutionary distances computed using the Tajima-Nei method [22] and are in the units of the number of base substitutions per site. Filled diamonds indicate morphologically and biochemically identified highly efficient Trichoderma isolates. Gen bank accession numbers for each ITS sequence has been given with the each morphologically identified Trichoderma isolate in the dendrogram.