S.no. Conc. of chemical mutagen (µg/ml) Isolate code Zone of starch hydrolysis (mm) Alpha-amylase activity (U/ml)
in Pikovskaya’s medium
1 50 HNO2-1 21 ± 2 910 ± 17
2 HNO2-10 35 ± 4 3967 ± 58
3 100 HNO2-2 29 ± 3 2433 ± 58
4 HNO2-20 25 ± 4 2833 ± 58
5 150 HNO2-3 22 ± 4 1213 ± 23
6 HNO2-30 33 ± 6 3833 ± 116
7 200 HNO2-4 33 ± 5 2617 ± 29
8 HNO2-40 24 ± 4 2033 ± 58
9 250 HNO2-5 21 ± 3 2233 ± 58
10 HNO2-50 25 ± 4 2017 ± 29
11 50 EMS-1 21 ± 2 2027 ± 46
12 EMS-10 33 ± 5 2367 ± 58
13 100 EMS-2 25 ± 4 2230 ± 52
14 EMS-20 21 ± 2 1567 ± 58
15 150 EMS-3 23 ± 5 1310 ± 17
16 EMS-30 28 ± 4 2825 ± 43
17 200 EMS-4 36 ± 2 4167 ± 58
18 EMS-40 24 ± 3 1313 ± 23
19 250 EMS-5 21 ± 5 1827 ± 46
20 EMS-50 27 ± 3 2167 ± 58
21 50 EtBr -1 26 ± 3 2233 ± 58
22 EtBr -10 21 ± 4 1023 ± 25
23 100 EtBr -2 28 ± 2 2367 ± 58
24 EtBr -20 27 ± 4 2433 ± 58
25 150 EtBr -3 29 ± 2 2767 ± 58
26 EtBr -30 21 ± 5 813 ± 23
27 200 EtBr -4 23 ± 2 1133 ± 58
28 EtBr -40 37 ± 3 4166 ± 58
29 250 EtBr -5 21 ± 5 1833 ± 58
30 EtBr -50 33 ± 5 4017 ± 76
31 50 Acr-1 33 ± 5 3950 ± 87
32 Acr-10 26 ± 2 2433 ± 58
33 100 Acr-2 25 ± 5 2833 ± 58
34 Acr-20 33 ± 5 4042 ± 72
35 150 Acr-3 22 ± 5 1333 ± 58
36 Acr-30 35 ± 5 3883 ± 29
37 200 Acr-4 33 ± 6 4237 ± 64
38 Acr-40 29 ± 2 2833 ± 58
39 250 Acr-5 39 ± 6 2833 ± 58
40 Acr-50 22 ± 4 1140 ± 53
41 50 5′-FU-1 30 ± 4 2783 ± 29
42 5 ׳ -FU-10 20 ± 5 1830 ± 52
43 100 5′-FU-2 21 ± 5 813 ± 23
44 5′-FU-20 27 ± 4 2577 ± 40
45 150 5′-FU-3 29 ± 2 2827 ± 46
46 5′-FU-30 30 ± 5 2810 ± 17
47 200 5′-FU-4 21 ± 5 717 ± 29
48 5′-FU-40 32 ± 5 2817 ± 58
49 250 5′-FU-5 20 ± 1 1227 ± 46
50 5′-FU-50 33 ± 5 3650 ± 50
Values presented in the table are means of triplicates ± SD
Table 2: Chemical mutant strains [Nitrous acid (HNO2), Ethyl methane sulfonate (EMS), Ethidium bromide (EtBr), Acrylamide (Acr) and 5 ׳-fluoro uracil (5 ׳-FU)] showing α-amylase potency in starch media and Pikovskaya’s medium.