Homologous protein Organism Identity (%) Positive (%) GenBank BACSU Bacillus subtilis 93 96 P10475 ERWCT Erwinia carotovora subsp. atroseptica 56 73 Q59394 BACS4 Bacillus sp. Strain N-4 56 71 P06566 CALSA Caldocellum saccharolyticum 44 60 P10474 DICD3 E. chrysanthemi strain 3937 41 58 P07103 RUMAL Ruminococcus albus 38 60 Q07940 BACS6 Bacillus sp. Strain KSM-635 35 52 P19424 PSEFL Pseudomonas fluorescens 34 47 P27033 CLOLO Clostridium longisporum 24 41 P54937 CLOSF Clostridium sp. strain F1 22 48 P23340 PAELA Paenbacillus lautus 21 42 P23550 PAEBA P. barcinonensis 20 47 O08342

*The amino acid sequences were compared using the NCBI’s BLAST search

Table 1: Comparisons of CMCase produced by E. coli JM109/LBH-10 with the glycosyl hydrolase family 5*.