Table 2

Run	X₁(g/L)	X₂(g/L)	X₃	Y₁(g/L)	Y₂ (U/mL)
1	75	5.0	8.0	5.99 ± 0.23	367.1 ± 25.2
2	50	7.5	7.0	5.71 ± 0.31	389.7 ± 33.6
3	50	7.5	7.0	5.82 ± 0.34	392.7 ± 28.4
4	50	7.5	7.0	5.86 ± 0.44	396.7 ± 35.8
5	25	10.0	8.0	5.21 ± 0.36	346.8 ± 29.7
6	75	10.0	8.0	6.19 ± 0.42	369.3 ± 30.2
7	8	7.5	7.0	4.72 ± 0.23	310.7 ± 28.7
8	50	3.3	7.0	5.49 ± 0.42	378.6 ± 33.9
9	25	10.0	6.0	5.15 ± 0.36	347.5 ± 30.8
10	25	5.0	6.0	5.06 ± 0.42	345.4 ± 28.7
11	50	7.5	8.7	5.29 ± 0.28	386.2 ± 35.2
12	75	10.0	6.0	6.12 ± 0.42	370.0 ± 25.6
13	50	7.5	7.0	5.66 ± 0.33	387.7 ± 29.5
14	50	7.5	5.3	5.22 ± 0.24	386.3 ± 36.3
15	50	7.5	7.0	5.60 ± 0.34	381.5 ± 42.5
16	92	7.5	7.0	6.81 ± 0.42	363.5 ± 33.8
17	25	5.0	8.0	5.12 ± 0.31	344.6 ± 29.7
18	50	7.5	7.0	5.52 ± 0.43	382.5 ± 28.2
19	75	5.0	6.0	6.21 ± 0.44	367.9 ± 32.7
20	50	11.7	7.0	5.65 ± 0.28	383.5 ± 33.6

Table 2: Central composite design (CCD) and determined response values.