Source of variation

Degree of freedom

Sum of squares

Mean squares

F-value

Probe>F

 

 

Cell growth

Model

9

4.45

0.49

26.29

<0.0001

X1

1

4.10

4.10

217.87

<0.0001

X2

1

0.02

0.02

1.22

0.2961

X3

1

0.00

0.00

0.03

0.8661

X12

1

0.03

0.03

1.78

0.2117

X22

1

0.00

0.00

0.33

0.5792

X32

1

0.25

0.25

13.35

0.0044

Error

5

0.08

0.02

-

-

Total

19

4.64

-

-

-

 

 

CMCase

Model

9

8307.01

923.00

17.63

<0.0001

X1

1

2338.26

2338.26

44.66

<0.0001

X2

1

20.52

20.52

0.39

0.5453

X3

1

0.78

0.78

0.02

0.9051

X12

1

5844.92

5844.92

111.64

<0.0001

X22

1

304.99

304.99

5.83

0.0365

X32

1

109.93

109.93

2.10

0.1780

Error

5

164.23

32.85

-

-

Total

19

8830.57

-

-

-

Table 3: Parameter estimates and analysis of variance (ANOVA) of the design for cell growth and production of CMCase by E. coli JM109/LBH-10.