Table 4

Run	X₁ (rpm)	X₂ (vvm)	Y₁ (g/L)	Y₂ (U/mL)
1	400	1.0	6.54 ± 0.47	572.7 ± 41.0
2	400	1.0	6.94 ± 0.58	542.9 ± 36.2
3	500	0.5	6.24 ± 0.52	472.3 ± 38.9
4	541	1.0	6.94 ± 0.54	571.4 ± 39.2
5	400	0.3	4.98 ± 0.38	334.4 ± 30.7
6	400	1.7	4.40 ± 0.45	373.5 ± 29.3
7	500	1.5	5.52 ± 0.36	488.2 ± 33.4
8	400	1.0	6.62 ± 0.42	531.7 ± 42.1
9	300	1.5	5.76 ± 0.44	384.1 ± 32.7
10	259	1.0	5.08 ± 0.39	397.9 ± 36.5
11	400	1.0	6.52 ± 0.41	537.3 ± 39.8
12	300	0.5	4.65 ± 0.47	388.1 ± 32.0
13	400	1.0	6.32 ± 0.51	568.6 ± 41.3

Table 4: Central composite design (CCD) and determined response values.