Source of variation Degree of freedom Sum of squares Mean squares F-value Probe>F   Cell growth Model 5 8.59 1.72 18.07 0.0007 X1 1 1.98 1.98 20.82 0.0026 X2 1 0.02 0.02 0.24 0.6370 X1·X2 1 0.84 0.84 8.80 0.0209 X12 1 0.40 0.40 4.24 0.0784 X22 1 5.65 5.65 59.36 0.0001 Error 4 0.20 0.05 - - Total 12 9.26 - - -     CMCase Model 5 90416.0 18083.2 42.84 <0.0001 X1 1 23508.3 23508.3 55.70 <0.0001 X2 1 564.4 564.4 1.34 0.2433 X1·X2 1 99.0 99.0 0.23 0.6101 X12 1 6482.5 6482.5 15.36 0.0042 X22 1 63946.1 63946.1 151.51 <0.0001 Error 4 1927.0 481.8 - - Total 12 93370.5 - - -

Table 5: Parameter estimates and analysis of variance (ANOVA) of the design for cell growth and production of CMCase by E. coli JM109/LBH-10.