| Scale |
Optimal condition |
E. coli JM109/LBH-10 [This study] |
P. aquimaris LBH-10 [13] |
| Cell growth |
CMCase |
Cell growth |
CMCase |
| Flask scale-1 |
Rice bran (g/L) |
73.4 |
57.1 |
- |
50.0 |
| Ammonium chloride (g/L) |
5.80 |
6.40 |
- |
3.0* |
| Initial pH |
6.54 |
6.70 |
- |
8.0 |
| Maximal production |
6.21 g/L |
388.9 U/mL |
- |
221.8 U/mL |
| Flask scale-2 |
Temperature (°C) |
40 |
35 |
25 |
30 |
| Maximal production |
6.12 g/L |
523.3 U/mL |
2.32 g/L |
334.6 U/mL |
| Bioreactor |
Agitation speed (rpm) |
480 |
480 |
400 |
300 |
| Aeration rate (vvm) |
1.0 |
1.0 |
1.5 |
1.0 |
| Maximal production |
6.81 g/L |
576.8 U/mL |
3.36 g/L |
320.3 U/mL |
|
| *Nitrogen source was peptone |
| Table 6: Comparison of optimal conditions for cell growth and production of CMCase by P. aquimaris LBH-10 and its recombinant E. coli JM109/LBH-10. |
