| Leaching parameter |
Xylanase activity (Ugds-1) |
Leaching parameter |
Xylanase activity (Ugds-1) |
| Leaching agents (1:10, w/v) |
|
Soaking time (min) |
|
| H2O |
2670.81 |
60 |
3305.21 |
| Methanol |
2150.16 |
90 |
4890.40 |
| Acetone |
2218.43 |
120 |
6290.10 |
| Butanol |
3500.80 |
150 |
5970.12 |
| Glycerol |
3902.24 |
180 |
4600.59 |
| NaCl (1.0%) |
5000.29 |
pH of extraction process |
|
| Citrate buffer (pH 4.0, 0.1 M) |
5708.51 |
3 |
5734.40 |
| Citrate buffer (pH 6.0, 0.1 M) |
5295.90 |
4 |
6290.10 |
| Phosphate buffer (pH 7.0, 0.1 M) |
3366.70 |
5 |
5585.90 |
| Glycine-NaOH buffer (pH 10.0, 0.1 M) |
560.84 |
6 |
4431.90 |
| Tween 80 (0.1%) |
5720.00 |
7 |
1600.30 |
| Tween 80 (0.2%) |
5812.04 |
8 |
1170.28 |
| Tween 80 (0.3%) |
5769.28 |
9 |
1054.22 |
| Citrate buffer (pH 4.0, 0.1 M) + Tween 80 (0.2%) |
6290.10 |
10 |
754.22 |
| Leaching agent: fermented substrate ratio (w /v) |
|
Temperature of extraction process (°C) |
|
| 1:2 |
1002.33 |
30 |
2216.65 |
| 1:4 |
1930.16 |
40 |
4372.10 |
| 1:6 |
4893.10 |
45 |
6290.10 |
| 1:8 |
6290.10 |
50 |
6312.45 |
| 1:10 |
6290.10 |
55 |
5396.00 |
| 1:12 |
6034.24 |
60 |
4153.07 |
| 1:20 |
5481.00 |
70 |
3305.19 |
| Soaking time |
|
Agitation mode (150 rpm) |
6312.45 |
| 30 |
2196. 74 |
Static mode |
3103.72 |
