| Purification step |
Total protein (mg) |
Xylanase activity (U) |
Specific activity (U/mg) |
Yield (%) |
Purification fold |
| Crude extract |
1986.50 |
21975.00 |
11.06 |
100 |
1 |
| (NH4)2SO4 fractionation (60-80%) |
671.00 |
17395. 42 |
25.93 |
79.16 |
2. 344 |
| DEAE- cellulose chromatography |
151.11 |
11736.86 |
77.67 |
53.41 |
7.021 |
| Sephadex G-200 chromatography |
23.62 |
9088.65 |
384.79 |
41.36 |
34.784 |
| Sephadex G-100 chromatography |
14.48 |
7145.53 |
493.48 |
32.52 |
44.610 |
|
