Figure 2: The recorded biosurfactant activity by the most relevant four isolates in the presence of (C) coconut oil 2%, (P) phenol 0.01% and/or (Cy) cyclohexanol 0.025% or combinations thereof. The isolates were incubated for 6 days at 35 ± 2°C and agitation rate 180 rpm.*: Indicates significant difference from control column (Coconut oil 2%) at P-value<0.05 (one way ANOVA test). The biosurfactants activity was expressed as zone diameter based on the oil spread test (A) and Emulsification unit ml-1 based on the emulsification assay (B).