Figure 3: The recorded biosurfactant activity by isolate M2H2 1 using coconut oil 2% w/v with increasing concentrations of phenol (100-1500 mgl-1). The isolate was incubated for 6 days at 35 ± 2°C and agitation rate 180 rpm. The biosurfactants activity was expressed as (zone diameter) based on the oil spread test (A) and Emulsification unit ml-1 based on the emulsification assay (B).