Substrates Paraffin Oil 2% w/v Olive Oil 2% w/v Castor Oil 2% w/v Bitter Almond Oil 2% w/v Coconut Oil 2% w/v Cyclohexanol 0.025% w/v
Isolates Test  
M2H2 1 OST (cm) 0.6 ± 0 8.9 ± 0.9 3.2 ± 0.7 4.6 ± 0.5 3.1 ± 0.1 1.8 ± 0.3
EA (EU ml-1) 10 ± 2 67 ± 10 169 ± 16 61 ± 2 75 ± 6 10 ± 1
M2H2 3 OST (cm) 1.9 ± 0.3 4.5 ± 0.2 4.4 ± 0.7 3.1 ± 0.2 4.8 ± 1.1 0
EA (EU ml-1) 39 ± 8 94 ± 9 86 ± 7 47 ± 4 265 ± 5 34 ± 1
M2H2 4 OST (cm) 7.3 ± 0.6 5.5 ± 0.8 5.8 ± 0.5 3.6 ± 0.7 8.2 ± 0 0
EA (EU ml-1) 54 ± 10 43 ± 6 68 ± 0.3 31 ± 4 189 ± 7 42 ± 3
M2H2 7 OST (cm) 5.6 ± 0.8 3.7 ± 0 4 ± 0.4 4.8 ± 0.5 5.1 ± 0.8 0
EA (EU ml-1) 30 ± 4 44 ± 7 90 ± 17 12 ± 0 410 ± 4 15 ± 2
M2H2 8 OST (cm) 4.5 ± 0.9 6 ± 1.2 5.7 ± 0.8 5.8 ± 0.1 7.6 ± 0.2 0
EA (EU ml-1) 0.53 ± 0 66 ± 9 51 ± 5 44 ± 2 169 ± 16 21 ± 2
M2H2 10 OST (cm) 3.7 ± 0.5 7.2 ± 0.4 6 ± 0.6 8.2 ± 1.1 7.7 ± 0.9 0
EA (EU ml-1) 2 ± 0 39 ± 1 48 ± 7 29 ± 7 92 ± 3 38 ± 5
M2H2 14 OST (cm) 8.9 ± 8 11.3 ± 0.3 9.3 ± 0.4 9.8 ± 0.5 7 ± 1 0
EA (EU ml-1) 13 ± 1 78 ± 8 24 ± 3 338 ± 53 31 ± 4 33 ± 4
M2H2 15 OST (cm) 2.4 ± 0 7.6 ± 0.2 5.8 ± 0.7 9.8 ± 0.1 6.5 ± 1.1 0
EA (EU ml-1) 21 ± 3 62 ± 5 38 ± 4 356 ± 33 21 ± 0.3 17 ± 3
M2H2 16 OST (cm) 0 7.1 ± 0.7 6.4 ± 0.9 2.8 ± 0 6.7 ± 0.4 0
EA (EU ml-1) 23 ± 2 58 ± 9 38 ± 3 52 ± 6 16 ± 1 13 ± 3
M2H2 18 OST (cm) 0 8.5 ± 0.3 7.1 ± 0.1 5.9 ± 1.3 9.6 ± 1.2 0
EA (EU ml-1) 2 ± 0 93 ± 14 33 ± 6 42 ± 4 17 ± 4 17 ± 3
Table 2: The recorded biosurfactant activity according to oil spread test (OST) and emulsification assay (EA) using different substrates. Relevant isolates were incubated for 6 days at 35 ± 2°C.