Group Treatment G1. Standard (NaHCO3 -18.0 g/l, 0.017 M salinity, pH 9) G2. –100% Carbon deficiency (NaHCO3 - 0.0 g/l) G3. –75% Carbon deficiency (NaHCO3 - 4.5 g/l) G4. –50% Carbon deficiency (NaHCO3 - 9.0 g/l) G5. 0.2 M Salinity G6. 0.4 M Salinity G7. 0.6 M Salinity G8. 0.8 M Salinity G9. pH 6 G10. pH 7 G11. pH 10 G12. pH 11 Three replicate were made for each treatment group.

Table 1: Experiments to evaluate the effect of different stress conditions.