Figure 1: SDS PAGE and anti-PARP catalytic site immunoblotting of lymphocyte lysates. Panel A.(a, b), Coomassie (0.5%) stained gels of lymphocyte lysates; a’, b’, anti-PARP immunoblottings of the samples in a, b, respectively. In a’(1-3), the band of PARP was at 62 kDa, the molecular mass of PARP2 (native) for all three analysed samples. In b’ (1-3) the immunobands was above 113 kDa, indicating a large automodification of PARP. The shift of molecular mass accounted for more than hundred ADPribose molecules modifying the enzyme. Panel B. The immunopatterns in A, b’, before (1, 2, 3) and after (1’, 2’, 3’) alkali incubation of PAR-PARP. In 1’- 3’ poly-ADPribose-free PARP localizes at the molecular mass of native enzyme.