Figure 5: TAG production from corn stover hydrolysate by R. opacus MITXM-61 in batch-culture fermentations. (a) Hydrolysis of ensiling corn stover using a commercially available enzyme cocktail. A hundred grams of the pretreated dry biomass and 10 g of CTec2 enzyme were mixed with a desired volume of 0.002 N HCl to reach a total volume of 1 liter and a pH of 5.0. The reaction was carried out in a shaker at 45°C, 200 rpm. After 3 and 6 h of duration, additional 50 g of the pretreated dry biomass was added into the suspension twice. (b) Time course kinetics of TAG production on the corn stover hydrolysate in Bioengineeringbioreactor cultivations. MITXM-61 was inoculated in the hydrolysate containing 118 gl-1 of total sugars composed of 82 gl-1 glucose, 32 gl-1 xylose and 4 gl-1 arabinose supplemented with 6.7 gl-1 (NH4)2SO4 and mineral components of defined medium. (c) Fatty acids composition profile as % of total fatty acids (g g-1) of TAGs from R. opacus MITXM-61 cells growing in the corn stover hydrolysate for 5 days in (b) and defined medium containing xylose for 5 days in Figure 2c or xylose/glucose mixture for 3 days in Figure 3. Data are results of triplicate experiments, ± s.d.