Figure 4: A) Reverse-phase liquid chromatography of the major carotenoids of Halorubrum sp. TBZ126 in optimized condition for cell growth under light. Column: 100-5 C18 column (300 by 4.6 mm, Knauer, Germany). Eluent: acetonitrile-dichloromethane-methanol (70:20:10 v/v/v), 20 mM ammonium acetate, and 0.1% triethylamine. Flow rate: 0.8 ml/min. Detection: 470 nm. Peak identities: peak 1, bacterioruberin; peak 2, all-trans-lycopene; peaks 3, 13-cis-lycopene; peak 4, alltrans- ββ-carotene. B) Mass spectra (ESI mode) of major carotenoids fractions from Halorubrum sp. TBZ126. Bacterioruberin (a), lycopene (b) and β-carotene (c). C) UV-VIS spectrum of total carotenoid extract from Halorubrum sp. TBZ126 in optimized condition for cell growth under light with typical characteristics of three fingered (1, 2 and 3) and two cis maxima (4 and 5) which corresponded to bacterioruberin [24].