Figure 5: A) Reverse-phase liquid chromatography of the major carotenoids of Halorubrum sp. TBZ126 in carotenoid production optimized condition and under light exposure. Column: 100-5 C18 column (300 by 4.6 mm, Knauer, Germany). Eluent: acetonitrile-dichloromethane-methanol (70:20:10 v/v/v), 20 mM ammonium acetate and 0.1% triethylamine with flow rate of 0.8 ml/min and detection at 470 nm. Peak identities: peak 1, bacterioruberin; peak 2, all-trans-lycopene; peaks 3, 13-cis-lycopene; peak 4, all-trans-β-carotene. B) UV-VIS spectrum of the total carotenoid extract from Halorubrum sp. TBZ126 in optimized condition for carotenoid production and under light exposure with typical characteristics of three fingered (1, 2 & 3) and two cis maxima (4 & 5) maximum absorbance.