Experimental variables Responses
Run x1 (temperature) x2 (pH) x3 (salinity) Biomass Volumetric
Carotenoid (mg/l)
Cellular Carotenoid (mg/g)
  Level °C Level   Level   NaCl (% w/v)
1 -1 22 1 8.7 1 24.43 0.48 5.27 10.98
2 0 32.5 0 7 0 16.25 0.82 10.1 12.32
3 -1 22 -1 5.3 -1 8.07 0.14 0.24 1.71
4 0 32.5 1.68 10 0 16.25 0.42 10.53 25.07
5 0 32.5 0 7 0 16.25 0.84 10.78 12.83
6 1 43 1 8.7 1 24.43 0.56 5.28 9.43
7 -1 15 0 7 0 16.25 0.35 0.83 2.37
8 0 32.5 0 7 -1.68 30 0.53 9.91 18.7
9 1 43 1 8.7 -1 8.07 0.15 0.89 5.93
10 1 43 -1 5.3 -1 8.07 0.04 0.19 4.75
11 0 32.5 0 7 0 16.25 0.83 10.43 12.57
12 -1.68 50 0 7 0 16.25 0.09 0.24 2.67
13 0 32.5 0 7 -1.68 2.5 0.05 0.96 19.2
14 -1 22 1 8.7 -1 8.07 0.14 1.03 7.36
15 -1 22 -1 5.3 1 24.43 0.13 1.32 10.15
16 0 32.5 0 7 0 16.25 0.84 10.78 12.83
17 0 32.5 0 7 0 16.25 0.83 10.53 12.69
18 0 32.5 -1.68 4 0 16.25 0.04 0.15 3.75
19 1 43 -1 5.3 1 24.43 0.12 1.1 9.17
20 0 32.5 0 7 0 16.25 0.84 10.78 12.83
Table 1: Central composite design (CCD) matrix (Experimental variables and responses).