Source of variation DF1) Sum of squares Mean square F-value p-value
The model for biomass production (Y1)2)
Regression 9 1.98 0.22 89.59 0
Linear 3 0.38 0.16 66.86 0
Square 3 1.53 0.51 207.81 0
Interaction 3 0.07 0.02 8.97 0.003
Residual error 10 0.02 0    
Lack-of-fit 5 0.02 0 72.6 0
Pure error 5 0 0    
Total 19 2      
Model terms Coefficient DF Standard error t-value p-value
Intercept -4.75 1 0.38 -12.62 0
x1 (temperature) 0.11 1 0.01 10.37 0
x2 (pH) 0.85 1 0.07 12.51 0
x3 (salinity) 0.06 1 0.01 4.66 0.001
x12 (temperature * temperature) -0.002 1 0 -16.47 0
x22 (pH * pH) -0.07 1 0.004 -16.2 0
x32 (salinity * salinity) -0.003 1 0 -14.56 0
x1 x2 (temperature * pH) 0.001 1 0 1.43 0.184
x1 x3 (temperature * salinity) 0 1 0 1.14 0.28
x2 x3 (pH * salinity) 0.006 1 0.001 4.85 0.001
1) Degree of freedom
2) R2=98.77% and adjusted R2 (R2 adj)=97.67%
Table 2: Analysis of variance (ANOVA) for biomass production by Halorubrum sp.TBZ126.