| Source of variation |
DF1) |
Sum of squares |
Mean square |
F-value |
p-value |
| The model for total carotenoid production (Y2)2) |
|
|
|
|
|
| Regression |
9 |
405.65 |
45.07 |
17.03 |
0 |
| Linear |
3 |
107.37 |
40.55 |
15.32 |
0 |
| Square |
3 |
292.9 |
97.63 |
36.89 |
0 |
| Interaction |
3 |
5.39 |
1.8 |
0.68 |
0.585 |
| Residual error |
10 |
26.79 |
2.65 |
|
|
| Lack-of-fit |
5 |
30.47 |
5.22 |
69.71 |
0 |
| Pure error |
5 |
0.37 |
0.07 |
|
|
| Total |
19 |
432.12 |
|
|
|
| Model terms |
Coefficient |
DF |
Standard error |
t-value |
p-value |
| Intercept |
-72.31 |
1 |
12.37 |
-5.85 |
0 |
| x1 (temperature) |
2.27 |
1 |
0.36 |
6.38 |
0 |
| x2 (pH) |
9.54 |
1 |
2.24 |
4.24 |
0.002 |
| x3 (salinity) |
0.85 |
1 |
0.41 |
2.06 |
0.066 |
| x12 (temperature * temperature) |
-0.03 |
1 |
0 |
-9.007 |
0 |
| x22 (pH * pH) |
-0.68 |
1 |
0.13 |
-5.04 |
0.001 |
| x32 (salinity * salinity) |
-0.03 |
1 |
0 |
-4.72 |
0.001 |
| x1 x2 (temperature * pH) |
0.005 |
1 |
0.03 |
0.17 |
0.867 |
| x1 x3 (temperature * salinity) |
0 |
1 |
0 |
-0.07 |
0.948 |
| x2 x3 (pH * salinity) |
0.05 |
1 |
0.04 |
1.41 |
0.188 |
|
| 1) Degree of freedom |
| 2) R2=93.87% and adjusted R2 (R2 adj)=88.36% |
| Table 3: Analysis of variance (ANOVA) for total carotenoid production by Halorubrum sp. TBZ126. |
