A. Replication phase standard curves. S. aureus 8325-4, DU5883, DU5723, and the MRSA arthroplasty isolate cultured in TSB with incorporated tetracycline and erythromycin for S. aureus DU5883. Assays were performed in triplicate and the mean absorbance at 490 nm (A490) with the standard error was plotted against culture time. B. Viable cell number standard curves. Cell density (CFU) and ATP-bioluminescence emitted from serially diluted standardised cultures of S. aureus 8325-4, DU5883, DU5723 (B1) and the MRSA arthroplasty isolate (B2) cultured in TSB with incorporated tetracycline and erythromycin for S. aureus DU5883. Assays were performed in triplicate and the mean relative light units (RLU) at 595nm (A595) with standard error was plotted against average CFU (error bars are shown in black on all graphs, but are difficult to see in some cases on account of the errors being small).
