A. Adherence of standardised cultures of S. aureus 8325-4, DU5883 (FnBPA/ B-deficient mutant), DU5723 (Protein A-deficient mutant), and MRSA to rabbit plasma-conditioned stainless steel coupons, with or without prior antiserum exposure (working titre of 1000, controls sterile deionised H2O).
B. Normalised mean adherence change expressed as a percentage of the adherence in presence of pre-immune serum to plasma-conditioned or unconditioned stainless steel coupons for S. aureus 8325-4, DU5883 and MRSA with exposure to rFnBP-A post-immune or purified IgG post-immune serum. All results were statistically significantly different to pre-immune serum exposure. C. Normalised mean adherence change expressed as a percentage of the adherence in presence of pre-immune serum to plasma-conditioned stainless steel coupons for S. aureus 8325-4 and DU5723 with exposure to rFnBP-A post-immune, rFnBP-A purified IgG post-immune serum or control purified IgG serum (*denotes a statistically significant p<0.05 difference to pre-immune serum exposure).
In all cases adherence was quantified by ATP-bioluminescence and culture. Assays were performed in triplicate and values represent the mean adherent CFU ml-1 or mean % adherent CFU ml-1 with standard error of pooled data (n>100 per parameter, error bars are shown in black). Sample means were compared by un-paired t-test adjusted for equal or unequal sample variances, significance level was p<0.05.
