A. Replication phase standard curves. S. aureus SH1000 and S. aureus SRC005 cultured in TSB, iron-limited, or iron replenished CL medium (CL, CL FeCl3) with incorporated erythromycin for culture of S. aureus SRC005. Assays were performed in triplicate and the mean absorbance at 490 nm (A490) with the standard error plotted against culture time. B. Viable cell number standard curves. Cell density (CFU) and ATP-bioluminescence emitted from standardised cultures of S. aureus SH1000 in TSB, CL, or CL FeCl3 medium. Assays were performed in triplicate and the mean relative light units (RLU) at 595 nm (A595) with standard error plotted against average CFU (error bars are shown in black on all graphs, but are difficult to see in some cases on account of the errors being small).
