A. Adherence of standardised cultures of S. aureus SH1000 and SRC005 (IsdA-deficient mutant) to rabbit plasma-conditioned stainless steel coupons, with or without prior antiserum exposure (working titre of 1000, controls sterile deionised H2O). B. Normalised mean adherence change expressed as a percentage of the adherence in presence of pre-immune serum to plasmaconditioned stainless steel coupons for S. aureus SH1000 and SRC005 with exposure to rIsdA post-immune or purified IgG post-immune serum. All results were statistically significantly different to pre-immune serum exposure. In all cases adherence was quantified by ATP-bioluminescence and culture. Assays were performed in triplicate and values represent the mean adherent CFU ml-1 or mean % adherent CFU ml-1 with standard error of pooled data (n>100 per parameter, error bars are shown in black). Sample means were compared by un-paired t-test adjusted for equal or unequal sample variances, significance level was p<0.05.
