| Strain |
Properties |
| Staphylococcus aureus |
| 8325-4a |
Wild type strain (rsbU - mutant) expressing FnBP-A, FnBP-B. NCTC 8325 cured of prophages [43]. |
| DU5883a |
FnBP-A, FnBP-B –deficient mutant of 8325-4 (fnbA::tet fnbB::ery) [44]. Tetracycline and Erythromycin resistance. |
| DU5723a |
Protein-A –deficient mutant of 8325-4 (Δspa-600::EtBr) [45]. |
| MRSAb |
Clinical Meticillin resistant S. aureus arthroplasty isolate. |
| SH1000c |
Wild type 8325-4 derivative strain expressing IsdA (rsbU+) [46]. |
| SRC005c |
IsdA-deficient mutant of SH1000 (isdA::Tn917 ery) [40]. Erythromycin resistance. |
| Escherichia coli |
| 514a |
E. coli XL1-Blue RecAˉ harbouring plasmid pQE30 – fnbA D1-4 [47,48]. Ampicillin resistance. |
| BL21 (DE3) pSRC00c |
E. coli BL21 (DE3) harbouring plasmid pET24d – isdA [40]. Kanamycin resistance. |
aStrains donated by Professor T. Foster, Trinity College, Dublin, Ireland. Cloning of fnbA D1-4 has been previously described [47,48]. bStrains donated by Southport and Ormskirk Hospital, Merseyside, UK. cStrains donated by Professor S. Foster, University of Sheffield, UK. Cloning of isdA has been previously described [40]. |
