Figure 5: Three-dimensional confocal microscopic images of fungal conidia and hypha of T. mentagorophyte after treatment with plasma. (a) Fungal conidia for control. (b) Fungal conidia threated with plasma for 3 minutes. (c) Fungal hypha for control. (b) Fungal hypha threated with plasma for 3 minutes. Plasma-treated samples were stained with FDA and PI and three-dimensional images of the fungal conidia and hypha were taken using a laser scanning confocal microscope (LSM 700) with a 10x objective lens and fluorescence optics (excitation at 488 nm for FDA and at 530 nm for PI, and emission at 520 nm for FDA and at 620 nm for PI). The three-dimensional images were analyzed using the ZEN imaging software