Figure 2: SDS-PAGE after staining with blue Coomassie and zymogram. (A) SDS-Page (15%): lane 1, partially purified sample by ammonium sulphate precipitation; lane 2-purified amylase after anion exchange chromatography; lane 3-purified amylase after gel filtration chromatography; lane S-standard protein markers (LMW); B) lane 3-zymogram of purified amylase after gel filtration chromatography.