Additives and Inhibitors Concentration Relative enzyme activity () Control* 0 100 EDTA 5 mM 35.68 10 mM 33.92 UREA 2 M 67.28 4 M 37.68 N-bromosuccinimmide 10 mM 0 β-mercaptoethanol 10 mM 104.88 20 mM 108.72 SDS 0.001 99.52 0.002 111.76 Triton X-100 0.001 99.84 0.002 109.52 Additives and Inhibitors Concentration Relative enzyme activity () Control* 0 100 EDTA 5 mM 35.68 10 mM 33.92 UREA 2 M 67.28 4 M 37.68 N-bromosuccinimmide 10 mM 0 β-mercaptoethanol 10 mM 104.88 20 mM 108.72 SDS 0.001 99.52 0.002 111.76 Triton X-100 0.001 99.84 0.002 109.52

*Control: enzyme activity measured in the absence of additives and inhibitors, assumed as 100

Table 5: Effect of various additives and inhibitors on the activity of α-amylase.