Figure 1: Schematic diagram of the primer positions in the [NiFe] hydrogenase gene of D.vulgaris. The following primer pairs were used to amplify the [NiFe] hydrogenase gene: Hyd2F-Hyd7R, Hyd1F- Hyd7R, Hyd4F-Hyd7R, Hyd2F-Hyd5R, Hyd1F-Hyd5R. In addition, Hyd4F was labelled with DIG and used as a probe in hybridization experiments to identify amplified hydrogenase sequences.