Figure 3: The relationship between PMA concentration and enumeration of E.faecalis using qPCR for the 16S rRNA gene and standard culture method on TSA media for A) heat-killed cells; and B) live cells. The initial cell concentration was approximately 108 CFU ml-1. The detection limit for qPCR is 3 x 102 GE ml-1 in natural water without pre-concentration.