Figure 4:The relationship between PMA concentration and enumeration of (A) heat-killed E.faecaliscells and (B) live E.faecalis cells, using qPCR for 16S rRNA gene and pbp5 gene, and standard culture method on TSA media. The initial cell concentration was diluted to approximately 106 CFU ml-1. The detection limit for qPCR is 3 x 102 GE ml-1 in natural water without preconcentration.