| Target gene |
Primer/probe |
Sequence (5’-3’) |
Final concen-tration (μM) |
Reference |
| Enterococcus spp. (23S rRNA gene) |
Forward primer |
GAGAAATTCCAAACGAACTTG |
0.5 |
|
| Reverse primer |
CAGTGCTCTACCTCCATCATT |
0.5 |
| Probe |
FAM -TGGTTCTCTCCGAAATAGCTTTAGGGCTA- TAMRA |
0.1 |
| E.faecalis
(16S rRNA gene) |
Forward primer |
CGCTTCTTTCCTCCCGAGT |
0.9 |
|
| Reverse primer |
GCCATGCGGCATAAACTG |
0.9 |
| Probe |
FAM – CAATTGGAAAGAGGAGTGGCGGACG - TAMRA |
0.25 |
| E.faecalis
(pbp5 gene) |
Forward primer |
AGGGACATTGAAGCCAGATG |
0.5 |
[18] |
| Reverse primer |
AAGCGGTACGCAGATTGACT |
0.5 |
| Probe |
FAM - TGTCGCAAAATAGCCGCCCCAAGATT - TAMRA |
0.1 |
| Salmon DNA
Sketa |
Forward primer |
GGTTTCCGCAGCTGGG |
0.5 |
|
| Reverse primer |
CCGAGCCGTCCTGGTCTA |
0.5 |
| Probe |
FAM – AGTCGCAGGCGGCCACCGT - TAMRA |
0.1 |
