Extraction method DNA puritya Lowest detection limit (GE reaction-1)a qPCR calibration curve [% Efficiency] Wizard® Genomic DNA Purification Kit 2.01 ± 0.05 12.95 ± 0.49 y = -3.6129x + 36.209 [E = 89%] Conventional 1.56 ± 0.06 13.95 ± 1.77 y = -3.0839x + 36.902 [E = 111%] QIAamp® DNA Mini Kit 1.92 ± 0.03  9.50  ± 0.71 y = -3.5611x + 32.869 [E = 91%] Notes: aThe values are averages ± standard errors based on two independent extractions

Table 2: Comparison of three DNA extraction methods from PBS spiked with pure cultures of E. faecalis.