Figure 3: Electrophoretic analysis of L-asparaginase produced by Aspergillus sp. ALAA-2000 at various stages of purification. M, molecular weight markers; (135.0, 100.0, 75.0, 63.0, 48.0, 35.0, 25.0 and 5.0 kDa); lane 1, crude-enzyme extracts; lane 2, fractional precipitation by (NH4)2SO4 80%; and lane 3, partial purified L-asparaginase on Sephadex G-200.