Substrate Leaching agent Hot water 40°C Glycine Tween 80 Citrate Sugar canebagasse Wheat bran Corn cobs Soy bean Kidney bean bran Wheat hay Corn casing Rice bran 14.59 10.21 8.22 23.34 9.33 0.73 0.29 2.92 2.04 0.15 0.15 1.31 1.02 1.89 1.75 1.46 18.38 22.76 3.65 18.97 13.57 6.62 9.78 21.45 15.61 15.03 8.61 17.51 22.03 0.73 9.34 4.38

Table 1: Production of L-asparaginase under solid state fermentation (SSF) and extracted with different leaching agents.