Purification Step Total activity (U) Total protein (mg) Specific activity (U/mg) Yield (%) Fold
Filtrate
(NH4)2SO4 precipitation
Sephadex G-200
30
24.6
13.1
620
457.4
32.3
0.048
0.053
0.4
100
82
43.6
1
1.11
8.3
Table 4: Summary of the purification steps of L-asparaginase.