Assays

Contrast coefficients

Atrazine degradation (%)

X1

X2

X3

X4

X5

X6

X7

X8

5 days

10 days

15 days

20 days

1

-1

-1

-1

-1

-1

-1

-1

-1

8.5

38.5

38.8

33.5

2

1

-1

-1

-1

1

1

1

-1

11.6

21.5

16.0

21.5

3

-1

1

-1

-1

1

1

-1

1

15.5

23.2

22.2

23.7

4

1

1

-1

-1

-1

-1

1

1

22.6

31.7

49.7

59.7

5

-1

-1

1

-1

1

-1

1

1

23.2

24.4

43.3

49.4

6

1

-1

1

-1

-1

1

-1

1

19.4

23.3

21.0

33.1

7

-1

1

1

-1

-1

1

1

-1

32.5

34.6

35.7

31.5

8

1

1

1

-1

1

-1

-1

-1

22.0

38.2

39.8

28.9

9

-1

-1

-1

1

-1

1

1

1

6.8

16.3

18.7

18.7

10

1

-1

-1

1

1

-1

-1

1

14.3

19.0

36.1

37.1

11

-1

1

-1

1

1

-1

1

-1

23.6

36.5

52.1

63.3

12

1

1

-1

1

-1

1

-1

-1

4.9

52.5

71.0

71.0

13

-1

-1

1

1

1

1

-1

-1

21.4

25.1

34.9

35.7

14

1

-1

1

1

-1

-1

1

-1

5.1

5.7

8.9

6.3

15

-1

1

1

1

-1

-1

-1

1

24.0

26.6

33.0

32.2

16

1

1

1

1

1

1

1

1

15.4

18.2

22.9

23.9

17

0

0

0

0

0

0

0

0

15.0

19.9

20.1

26.0

18

0

0

0

0

0

0

0

0

21.6

24.9

25.9

38.5

19

0

0

0

0

0

0

0

0

23.5

26.9

28.1

23.6

20

0

0

0

0

0

0

0

0

16.0

19.6

18.1

24.8

X1 – ZnSO4 (g L-1): (-1): 0.0, (0): 0.001, (+1): 0.002
X2 - FeSO4: (g L-1) (-1): 0.0, (0): 0.0005, (+1): 0.001
X3 - MnSO4 (g L-1): (-1): 0.0, (0): 0.05, (+1): 0.1
X4 - MgSO4 (g L-1): (-1): 0.0, (0): 0.5, (+1): 1.0
X5 - CuSO4 (g L-1): (-1): 0.0, (0): 0.25, (+1): 0.5
X6 – glucose (g L-1): (-1): 2.0, (0): 5.0, (+1): 8.0
X7 – peptone (g L-1): (-1): 1.0, (0): 3.0, (+1): 5.0
X8 – yeast extract (g L-1): (-1): 2.0, (0): 5.0, (+1): 8.0
Table 1: Fractional factional design 2IV 8-4 with encoded variables and atrazine degradation.