Conditions studied Culture medium Composition (g L-1) Pergentage of degradation after 10 days Pergentage of degradation after 15 days Initial Condition [30] ZnSO4 (0.001) FeSO4 (0.0005) MnSO4 (0.05) MgSO4 (0.5) CuSO4 (0.25) Glucose (5.0) Peptone (3.0) Yeast Extract (5.0) 22.8 39.0 Fractional factorial design (2IV 8-4) (Best condition – assay 12) ZnSO4 (0.002) FeSO4 (0.001) MgSO4 (1.0) Glucose (8.0) Peptone (1.0) Yeast Extract (2.0) 52.5 71.0 Full experimental design (32) (Best condition – assay 7) FeSO4 (0.001) MnSO4 (0.05) Glucose (2.0) Peptone (1.0) Yeast Extract (2.0) 90.3 94.5

Table 4: Comparative analyses of culture media composition before and after the experimental design. The best results of atrazine degradation were also given.