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Figure 3: The effect of increasing CCCP and C. emberbe extract on the efflux of ciprofloxacin from M. smegmatis. The compounds were added at various concentrations to the cells. The cells were then centrifuged and the amount ciprofloxacin in the supernatant quantified at excitation and emission wavelengths of 270 nm and 452 nm respectively using an RF-1501 Shimadzu spectrofluorimeter. Values are means + SD for duplicate measurements. |